The TAR DNA binding protein of 43 kDa (TDP-43) is a ubiquitously expressed nuclear protein with roles in transcription and splicing regulation. A hyper-phosphorylated, ubiquitinated and cleaved form of TDP-43, known as pathologic TDP-43 is the major disease protein in ubiquitin-positive, tau and α-synuclein-negative frontotemporal dementia (FTLD-TDP, previously referred to as FTLD-U) and in amyotrophic lateral sclerosis (ALS). Evidences indicates that TDP-43 can be detected in human plasma and CSF and levels are reportedly elevated in cases of ALS, FTLD and Alzheimer’s disease. ^1,2

The “MagQu” TDP-43 IMR Reagent is designed for rapid quantifying TDP-43 by ImmunoMagnetic Reduction (IMR). We conjugate antibody on the surface of around 50 nm-in-diameter Fe3O4 magnetic particles. When the antibodies on the surface bind with TDP-43, the magnetic particles form clusters. Therefore, the ac susceptibility (Xac) of magnetic particles would be reduced in the adding ac magnetic field. By measuring the reduction of Xac, TDP-43 can be easily, rapidly and accurately quantified.

References

1. Foulds, P., McAuley, E., Gibbons, L., Davidson, Y., Pickering-Brown, S. M., Neary, D., Snowden, J. S., Allsop, D., Mann, D. M. (2008). TDP-43 protein in plasma may index TDP-43 brain pathology in Alzheimer's disease and frontotemporal lobar degeneration. Acta neuropathologica, 116(2), 141-6.
2. Feneberg, E., Gray, E., Ansorge, O., Talbot, K., & Turner, M. R. (2018). Towards a TDP-43-Based Biomarker for ALS and FTLD. Molecular neurobiology, 55(10), 7789-7801.