Product
Qbeads-NTA-Ni
- Catalog No. MF-HIS-3000
- Purification of 6xHis tagged protein by easy magnetic purification.
Features:
- Easy to use
- Fast operation without centrifugation
- Good magnetism and high hydrophilic
- Small scale (~μg) to large scale (~mg) protein purification
Description
Qbeads-NTA-Ni is designed for rapid purification of 6xHis-tagged proteins. Qbeads-NTA-Ni has nitrilo- triacetic acid (NTA) groups with charged nickel covalently bind to surface dextran of Qbeads. Due to the high affinity, Qbeads-NTA-Ni can be used for capturing 6xHis-tagged proteins. Bound 6xHis-tagged proteins can be temporarily immobilized under magnetic attraction, so the other parts in supernatant can be removed easily and efficiently. Bound proteins can be directly used in downstream applications or be eluted off the beads. The capacity of purified 6xHis-tagged proteins (~35kDa) captured by Qbeads-NTA-Ni is approximately 5 mg/mL.
Specification:
Core
Iron Oxide (Fe3O4)
Layer
Dextran
Surface protein
NTA-Ni
Mean Diameter of Particles
~1 μm
Storage Buffer
20% ethanol
Storage temp.
2 ~ 8 ℃
Capacity
5 mg/mL
Ordering information:
| Catalog Number | Description | Package Size |
|---|---|---|
| MF-HIS-3000-2ML | - | 1 mL x 2 |
| MF-HIS-3000-5ML | - | 1 mL x 5 |
| MF-HIS-3000-10ML | - | 1 mL x 10 |
