Product

Carcinoembryonic Antigen (CEA) IMR Reagent

  • Catalog No. MF-CEA-0061
  • For rapid quantifying CEA protein by Immuno Magnetic Reduction (IMR).
Features:
  • Quantify CEA protein in the sample easily, rapidly, and accurately
  • Magnetic Nanoparticle
  • Dextran layer
  • For Colorectal Cancer’s disease (CRC) research and for in-vitro diagnosis use
Description

Use Carcinoembryonic Antigen (CEA) IMR Reagent only with the XacPro-E System (MagQu Co., Ltd.). The reagent is used to quantitatively measure CEA protein in human fluid specimen, such as serum.

Human carcinoembryonic antigen (CEA) (MW = 175,000 – 200,000 g/mol) is a glycoprotein involved in cell adhesion. It is normally produced during fetal development, but the production of CEA stops before birth. Therefore, it is not usually present in the blood of healthy adults, although levels are raised in heavy smokers. Both benign and malignant (harmless and cancerous) conditions can increase the CEA level in blood. The most frequent cancer which causes an increased CEA concentration is cancer of the colon and rectum (Colorectal Cancer, CRC). The normal range for CEA concentration in an adult non-smoker is < 2.5 ng/ml and for a smoker < 5.0 ng/ml in blood.

CEA (ng/mL)

ColoRectal Cancer

> 10

High risk

2.5 (5.0) -10

Low risk

< 2.5 (5.0)

Normal (Smoker)

 

The Carcinoembryonic Antigen (CEA) IMR Reagent is designed for rapid quantifying CEA by Immuno Magnetic Reduction (IMR). We conjugated the antibody on the surface of around 50 nm-in-diameter Fe3O4 magnetic particles. When the antibodies on the surface bind with CEA, the magnetic particles form clusters. Therefore, the ac susceptibility (Xac) of magnetic particles would be reduced in the adding ac magnetic field. By measuring the reduction of Xac, we can quantify CEA protein in the sample easily, rapidly, and accurately.

Reference

  1. Perkins GL, Slater ED, Sanders GK, et al; Serum tumor markers. Am Fam Physician. 2003 Sep 15;68(6):1075-82.
  2. Duffy MJ; Carcinoembryonic antigen as a marker for colorectal cancer: is it clinically useful? Clin Chem. 2001 Apr;47(4):624-30.
  3. Hong, C.Y., Wu, C.C., Chiu, Y.C., Yang, S.Y., Horng, H.E., Yang, H.C., 2006. Appl. Phys. Lett. 88, 212512-1–212512-3.
  4. Che-Chuan Yang, Shieh-Yueh Yang, Chia-Shin Ho, et al.”Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: a feasibility study for clinical use.” Journal of Nanobiotechnology 2014, 12:44.
Specification:
Core
Iron Oxide (Fe3O4)
Layer
Dextran
Surface protein
Anti-CEA Antibody
Mean Diameter of Particles
50 ~ 60 nm
The analytical range of reagent
0.13 to 100 ng/mL CEA protein
Buffer
pH 7.4 PBS
Storage temp
2 ~ 8 ℃
Ordering information:
Catalog Number Description Package Size
MF-CEA-0061 100 tests 1 mL x 4
Applications:
  • Applications Content: 
    Quantitative detection for ultra-low-concentration bio-molecules, e.g. Colorectal Cancer’s disease (CRC) bio-markers in blood.